酶标抗体和酶-抗酶复合物的应用于免疫酶组化技术（APAAP法）
网页2010年12月2日 · 碱性磷酸酶-抗碱性磷酸酶桥联酶染色法(APAAP法)，是一种免疫组化技术。 用兔抗鼠IgG起搭桥作用，其中一个Fab段连接McAb ，另一个Fab段连接APAAP复合物， 再通过复合物中的碱性磷酸酶催化底物显色来判断抗原分子的存在。
Use of the APAAP Method in the Classification and Diagnosis of ...
网页1988年3月1日 · To circumvent this difficulty, an immunoalkaline phosphatase method (alkaline phosphatase anti - alkaline phosphatase, or APAAP) for labeling antigens, particularly in peroxidase-rich tissues, has been developed.7 Because endogenous alkaline phosphatase (AP) may also be present in many tissues (intestinal mucosa and proximal renal tubules) and ...
Peroxidase Antiperoxidase Complex - an overview - ScienceDirect
网页1983年1月24日 · In the immunocytochemical study, the peroxidase anti-peroxidase (PAP) method was used to investigate the fate of the lipoprotein inside the oocyte. A PAP complex, as produced by Vandesande (1978), and a specific antiserum against the lipoprotein fraction from the hemolymph of Sarcophaga bulloto were used.
免疫碱性磷酸酶组织化学实验——APAAP法 - antpedia
网页2019年4月6日 · 碱性磷酸酶抗碱性磷酸酶（APAAP）免疫组化染色技术. 实验方法原理APAAP（Alkaline phosphatase-anti-alkaline phosphatase technique）技术的基本原理为：抗鼠IgG抗体作为桥梁，将鼠源性识别细胞抗原的第一抗体与鼠源性的抗碱性磷酸酶单克隆抗体-碱性磷酸酶复合物相连接，使之成为Ag，-Ab1－Ab2- 2019-04-01 22:33 News WIKI 相关搜索. 免疫金组织化学染色实验——IGSS－法.
Developing Monoclonal Antibodies for Immunohistochemistry
网页2022年1月12日 · In 1983, we turned our attention towards using immune complexes of alkaline phosphatase monoclonal anti-alkaline phosphatase (APAAP complexes). The APAAP technique gave excellent immunocytochemical labelling reactions comparable in clarity and intensity to those obtained by immunoperoxidase procedures [ 4 ].
Use of the APAAP method in the classification and diagnosis of ...
网页The APAAP technique is a sensitive and relatively easy immunocytochemical method to perform. It requires only a modest amount of laboratory space and no expensive equipment with the exception of a light microscope. Staining can be performed on peripheral blood and bone marrow films as well as cryost …
碱性磷酸酶－抗碱性磷酸(APAAP)免疫组化染色技术检测淋巴细胞表
网页2013年12月26日 · 碱性磷酸酶－抗碱性磷酸 (APAAP)免疫组化染色技术检测淋巴细胞表. APAAP (Alkaline phosphatase-anti-alkaline phosphatase technique) 技术的基本原理为: 抗鼠IgG抗体作为桥梁，将鼠源性识别 细胞 抗原的第一抗体与鼠源性的抗碱性磷酸酶单克隆抗体-碱性磷酸酶复合物相连接，使 ...
APAAP complex: production and usage in immunocytochemical …
网页Objective: Production of APAAP complexes and comparing them with similar foreign products to determine the site of antigen in tissues or in cells. Methods: Secreted antibodies of the two hybridomas (A(1)G(9)G(3) and A(1)G(8)F(7) produced in our laboratory) were concentrated, purified and characterized.
网页The APAAP technique may be used on a variety of sample preparations. Unfixed surgical specimens embedded in OCT (Miles Scientific) or 7.5 per cent gelatin (275 bloom; Fisher Scientific) may be snap frozen in liquid nitrogen and
细胞表面标记的检测技术-碱性磷酸酶-抗碱性磷酸酶（APAAP）免 …
网页APAAP（Alkaline phosphatase-anti-alkaline phosphatase technique）技术的基本原理为：抗鼠IgG抗体作为桥梁，将鼠源性识别细胞抗原的第一抗体与鼠源性的抗碱性磷酸酶单克隆抗体-碱性磷酸酶复合物相连接，使之成为Ag，-Ab1－Ab2-anti AP-AP的复合物。. 还可通过二抗将APAAP 复合物 ...