Gram Stain: What It Is, Purpose, Procedure & Results - Cleveland Clinic
网页A Gram stain is a laboratory test that checks for bacteria at the site of a suspected infection or in certain bodily fluids. A medical laboratory scientist processes the Gram stain, which gives relatively quick results, so healthcare providers can know if bacteria are present, and, if so, the general type (s).
Clostridia: Sporeforming Anaerobic Bacilli - Medical Microbiology ...
网页Clostridia are strictly anaerobic to aerotolerant sporeforming bacilli found in soil as well as in normal intestinal flora of man and animals. There are both gram-positive and gram-negative species, although the majority of isolates are gram-positive. Exotoxin (s) play an important role in disease pathogenesis.
Gram Staining - StatPearls - NCBI Bookshelf - National Center …
网页2023年8月14日 — Gram stain is often the initial diagnostic test for the evaluation of infections. The use of Gram stain facilitates the rapid use of appropriate antibiotics. However, genetic sequences and molecular techniques …
Clostridioides difficile infection - StatPearls - NCBI Bookshelf
网页2024年4月10日 — Clostridioides difficile, formerly known as Clostridium difficile, is a gram-positive and spore-forming bacterium. This obligate anaerobic bacillus is recognized for its ability to produce toxins and cause diarrhea, which is often associated with antibiotic usage.
Approach to Gram stain and culture results in the ... - UpToDate
网页2024年7月31日 — Gram staining of clinical specimens (including sterile and nonsterile body fluid specimens, biopsy specimens, and positive culture specimens) is useful for guiding empiric clinical management for bacterial infections pending definitive culture data and/or molecular data.
Gram Staining: Principle, Procedure, Interpretation, Examples …
网页2022年8月10日 — Gram Staining is the common, important, and most used differential staining techniques in microbiology, which was introduced by Danish Bacteriologist Hans Christian Gram in 1884. This test differentiate the bacteria into Gram Positive and Gram Negative Bacteria, which helps in the classification and differentiations of microorganisms.
4.4 Gram-Positive Bacteria - Microbiology - OpenStax
网页Crystal violet, the primary stain of the Gram stain procedure, is readily retained and stabilized within this matrix, causing gram-positive prokaryotes to appear purple under a brightfield microscope after Gram staining. For many years, the retention of Gram stain was one of the main criteria used to classify prokaryotes, even though some ...
Clostridial Soft-Tissue Infections - Clostridial Soft-Tissue Infections ...
网页Gram stain and culture. Early suspicion and intervention are essential; clostridial cellulitis responds well to treatment, but myonecrosis has a mortality rate of ≥ 40% with treatment and 100% without treatment.
Clostridium difficile infection | Nature Reviews Disease Primers
网页2016年4月7日 — Infection of the colon with the Gram-positive bacterium Clostridium difficile is potentially life threatening, especially in elderly people and in patients who have dysbiosis of the gut...
网页The performance of the Gram Stain on any sample requires four basic steps that include applying a primary stain (crystal violet) to a heat-fixed smear, followed by the addition of a mordant (Gram’s Iodine), rapid decolorization with alcohol, acetone, or a mixture of alcohol and acetone and lastly, counterstaining with safranin.