Columns Used in HPLC: Different Types and How to Choose
网页2024年1月5日 · This article explores the different types of columns used in HPLC and provides a detailed introduction to chiral columns. Types of Columns Used in HPLC. 1. Normal-Phase Columns. Principle: Polar stationary phase and non-polar mobile phase. Applications: Separation of nonpolar compounds such as lipids, steroids, and fat-soluble …
How Do I Choose- A Guide to HPLC Column Selection
网页Modern column technology that offers higher performance at similar backpressure. or comparable performance at reduced backpressure. Designed in with Agilent LC instruments and supplies. Universal column platform with offerings for all separation modes, i.e., RP, NP, HILIC, SFC as well as chiral LC.
How HPLC Columns Work | Thermo Fisher Scientific - US
网页Learn more about the different HPLC column types and applications › Easily find the correct column with our digital LC column selection guide ›
12.5: High-Performance Liquid Chromatography - Chemistry …
网页2021年11月13日 · HPLC Columns. An HPLC typically includes two columns: an analytical column, which is responsible for the separation, and a guard column that is placed before the analytical column to protect it from contamination.
HPLC Basics | Thermo Fisher Scientific - US
网页An HPLC instrument has four major components: a pump to deliver the mobile phase, an autosampler to inject the sample, a stationary phase column to separate the sample compounds, and a detector to measure the compounds.
HPLC Columns - Bio-Rad
网页Find the right HPLC column for your analytical needs. Ion exchange, reverse-phase, and size exclusion HPLC columns in a variety of pore sizes and chemistry.
High-performance liquid chromatography - Wikipedia
网页High-performance liquid chromatography (HPLC), formerly referred to as high-pressure liquid chromatography, is a technique in analytical chemistry used to separate, identify, and quantify specific components in mixtures.
HPLC Columns Selection Guide: Types, Features, Applications
网页High performance liquid chromatography (HPLC) is a separation technique that uses liquid mobile phase to separate the components of a mixture. The sample is first dissolved in a solvent, then forced to flow through a chromatographic column under high pressure.
网页2016年2月1日 · compiled this handy guide to choosing LC columns, with plenty of tips and tricks to make your job easier and more productive. In addition, we’ve drawn on more than 40 years of experience to provide suggestions for
HPLC Column Types - Liquid Chromatography | Waters
网页Address challenging separations using Waters' analytical and preparative HPLC columns, available in over 5,800 column formats, 175 configurations, and 21 phases. Achieve exceptional performance and reproducibility across columns.
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