The Southern blot is the most common way to analyse restriction endonuclease-digested DNA to find specific sequences of interest. It uses the fact that denatured nucleic acids will bind to ...
The technique became known as 'northern blotting'—a pun ... proteins to nitrocellulose membranes 7, published later the same year. Today, Towbin's western method is clearly the preferred ...
Following separation, the proteins are transferred from the gel onto a blotting membrane. Although this step is what gives the technique the name "western blotting," the term is typically used to ...
Immunoassays that share common false reactivity should not be used in combination. A western blot is typically the final step in a diagnostic algorithm, because if correctly interpreted it is the ...
This valuable study reveals extensive binding of eukaryotic translation initiation factor 3 (eIF3) to the 3' untranslated regions (UTRs) of efficiently translated mRNAs in human pluripotent stem ...
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Western blotting forms the bedrock of many protein studies, with the technique cited in 9% of all protein related papers. The technique had its 40th birthday in 2019, an event that was widely ...
Western Lightning PLUS 化学发光试剂是一个基于增强型的luminol底物和HRP催化的检测系统。利用化学发光,可检测到1-10pg的蛋白,可以多重曝光。其灵敏度是一般试剂的2-8倍。适用于PVDF和硝化纤维素膜(nitrocellulose)。 Western Lightning化学发光试剂是一种快速的蛋白检测系统。
This important manuscript provides insights into the competition between Splicing Factor 1 (SF1) and Quaking (QKI) for binding at the ACUAA branch point sequence in a model intron, regulating exon ...