This protocol was adapted from Northern blotting: Transfer of denatured RNA to membranes. In Molecular Cloning: A Laboratory Manual (eds. Sambrook, J. & Russell, D.W.) 7.35–7.41 (Cold Spring ...
It is speculated that the presence of a bulk protein is necessary to prevent the DNA polymerase from becoming trapped and denatured in the oil/water interface of the emulsion droplets. In the case ...